Rhesus macaque17573 Exome sequencing

Genomic DNA was isolated from a rhesus macaque (Macaca mulatta), animal number 17573 (reference animal). A human exome kit, the SureSelect XT HumanAllExon 50Mb kit (G7544A), was used to pull down fragments containing exons. Fragments were sequenced on an Illumina HiSeq (2X101). This study is part of a project to improve the rhesus macaque reference assembly and annotation. The exomic data can also be used to identify the frequency of mutations in rhesus exons.

本研究从编号为17573的恒河猴（Macaca mulatta，参考动物）中分离得到基因组DNA。采用人类外显子组捕获试剂盒——SureSelect XT HumanAllExon 50Mb试剂盒（货号G7544A）——捕获包含外显子的DNA片段，随后在Illumina HiSeq测序平台上以2×101 bp双端测序模式完成测序。本研究是优化恒河猴参考基因组组装与注释项目的组成部分，该外显子组数据亦可用于检测恒河猴外显子区域的突变频率。