RNA-Seq of nucleus pulposus cells with or without CircFUNDC1 overexpression

RNA-Seq was used to detect the dwonstream genes after CircFUNDC1 overexpression. Method: Three nucleus pulposus cell total RNA profiles of Ad-NC samples and Ad-CircFUNDC1 samples were analyzed. Overall design: We collected three human nucleus pulposus samples and primary nucleus pulposus cells(NPCs) were cultured. Then, NPCs were transfected with CircFUNDC1 adenovirus (pAdeasy-EF1- hsa_circ_0007290-CMV-EGFP-N) or negetive adenovirus. After transfections, RNAs were collected and RNA-seq were performed

采用RNA测序（RNA-Seq）检测CircFUNDC1过表达后的下游基因。
方法：对Ad-NC样本与Ad-CircFUNDC1样本的髓核细胞总RNA表达谱进行分析，共3份髓核细胞总RNA表达谱。
总体设计：收集3份人髓核样本并培养原代髓核细胞（NPCs）；随后将原代髓核细胞分别转染携带CircFUNDC1的腺病毒（pAdeasy-EF1- hsa_circ_0007290-CMV-EGFP-N）或阴性对照腺病毒；转染完成后收集总RNA并开展RNA测序实验。