AFLP dataset

Amietia wittei and Amietia angolensis tissue samples were collected from sites on the southern slope of Mount Kilimanjaro, Tanzania, in 2011. Total genomic DNA was extracted with the Roche High Pure PCR Template Preparation Kit according to the manufacturer's protocol, except for an extra step after the digestion by proteinase K aiming to discard residual pigments. DNA was digested and fragments were amplified using six primer pair combinations using a modified version of Vos et al. (1995) (Appendix S1, Supporting Information). AFLP fragments were separated in an ABI 3130XL automatic capillary sequencer (Applied Biosystems) with an internal GeneScan 500 ROX size standard at the Department of Human Genetics, Biozentrum, University of Würzburg. AFLP peaks were determined using GeneMapper 4.1 (Applied Biosystems) and the final binary matrices were obtained following thw semi-automated scoring procedure described in Whitlock et al. (2008). The data include presence (1), absence (0), unscored (-1) AFLP fragments (in column) for each study site (rows) for Amietia wittei and Amietia angolensis separately.

2011年，研究人员于坦桑尼亚乞力马扎罗山南坡的多个采样位点采集了威氏安尼蛙（Amietia wittei）与安哥拉安尼蛙（Amietia angolensis）的组织样本。采用罗氏（Roche）High Pure PCR模板制备试剂盒，按照制造商操作规程提取总基因组DNA，仅在蛋白酶K（Proteinase K）消化步骤后增设了额外工序以去除残留色素。使用6对引物组合对酶切后的DNA片段进行扩增，所用方法为经改良的Vos等（1995）方案（详见附录S1及补充信息）。扩增片段长度多态性（Amplified Fragment Length Polymorphism，AFLP）片段在德国维尔茨堡大学生物中心人类遗传学系的ABI 3130XL型全自动毛细管测序仪（应用生物系统公司，Applied Biosystems）上完成分离，内参选用GeneScan 500 ROX分子量标准。采用GeneMapper 4.1软件（应用生物系统公司，Applied Biosystems）对AFLP峰图进行判读，最终通过遵循Whitlock等（2008）描述的半自动化评分流程得到二分型矩阵。本数据集分别针对威氏安尼蛙与安哥拉安尼蛙，构建了以采样位点为行、AFLP片段为列的矩阵，矩阵元素包含片段存在（1）、片段缺失（0）及未评分（-1）三种状态。