PTPσ Novel Object Recognition

<b>Novel Object Recognition test.</b> Male and female mice 3-4 months old were used for these experiments. NOR test was performed in a transparent arena (39x20x16 cm) where two identical objects (white plastic ping-pong balls glued to 50-ml Falcon tube caps) holders at the bottom) were located (adapted from (Casarotto et al., 2021)). The animals were allowed to explore the objects for 15 min in 3 consecutive days (training sessions). At the test session (4 hours or 5 days after the last training session to assess short-term or long-term memory, respectively) one of the old objects was replaced by a new one (black rubber squash ball glued to 50-ml Falcon tube caps), and the animals were allowed to explore both objects for 5 min. The number of interactions with the objects was calculated, and the difference between the visits to the new object and the old object (fB-fA) was used to assess memory. Total number of visits (fA+fB) was used to assess total exploratory activity. Two different cohorts of animals were used to assess short-term and long-term memory.

**新型物体识别实验（Novel Object Recognition test）**。本实验使用3-4月龄的雌雄小鼠。该实验在尺寸为39×20×16 cm的透明实验箱中开展，箱内底部放置两个完全相同的物体（固定于50ml Falcon管盖上的白色塑料乒乓球，实验范式改编自Casarotto等人2021年的研究）。实验动物需连续3天进行探索训练，每日探索时长为15分钟。在测试阶段（分别于末次训练后4小时或5天进行，以评估短时记忆或长时记忆），将其中一个原有物体替换为新型物体（固定于50ml Falcon管盖上的黑色橡胶壁球），随后允许实验动物探索两个物体共5分钟。统计实验动物与物体的互动次数，以新物体与原有物体的探索次数之差（fB-fA）评估记忆能力；以总探索次数（fA+fB）评估整体探索活跃度。本研究使用两批独立的实验动物队列分别评估短时记忆与长时记忆。