Poly(A)-capture full-length double-strand cDNA sequencing for transcriptome analysis

We tried two methods which are the DNase I treated full-length double-strand cDNA sequencing and the poly(A) capture full-length double-strand cDNA sequencing to avoid the non-specific genomic DNA amplification. Transcriptome analysis by Total RNA, DNaseI-treated and Poly(A) capture Sequencing.

为规避非特异性基因组DNA扩增，本研究尝试了两种测序方法：经脱氧核糖核酸酶I（DNase I）处理的全长双链互补脱氧核糖核酸（cDNA）测序，以及poly(A)捕获的全长双链cDNA测序。本研究通过总RNA测序、脱氧核糖核酸酶I处理测序及poly(A)捕获测序开展转录组分析。