FBXO5 degrades DOK6 to enhance cell mechanics and sensitivity to Temozolomide

stable CRISPR/Cas9 sgRNA-expression U251 cells were treated with 200nM or 1500nM temozolomide for 72 hours, and then, we screened temozolomide-resistance genes by negative selection and temozolomide-sensitive genes by positive selection Examination of differentially expressed sgRNAs in nagative group and positive group with control.

将稳定表达CRISPR/Cas9单引导RNA（sgRNA）的U251细胞以200nM或1500nM浓度的替莫唑胺（temozolomide）处理72小时；随后通过负筛选法筛选替莫唑胺耐药基因，正筛选法筛选替莫唑胺敏感基因，并以对照组为参照，对负筛选组与正筛选组中的差异表达sgRNA进行检测分析。