Metagenomic regulatory sequence library characterized in RpoD ortholog strains. Metagenomic regulatory sequence library characterized in RpoD ortholog strains

Characterization of a metagenomic regulatory sequence library derived from M. xanthus, E. coli, and O. urethralis genomes in strains expressing different RpoD ortholog variants. Targeted DNA and RNA seq used to profile relative DNA and RNA abundances, respectively of each regulatory sequence construct in the library.

本研究针对表达不同RpoD同源变体的菌株所构建的、源自黄色粘球菌（Myxococcus xanthus）、大肠杆菌（Escherichia coli）与尿道奥尔森菌（Olsenella urethralis）基因组的宏基因组调控序列文库开展表征分析。实验分别采用靶向DNA测序与靶向RNA测序技术，对该文库中各调控序列构建体的DNA与RNA相对丰度进行定量检测。