TGF-ß Signaling Pathway and Cell-Cell Interactions in the Tumor Microenvironment Drive Bladder Cancer Progression after BCG Instillation Treatment: Insights from longitudinal Single-Nucleus RNA-Seq

In this study, we aimed to characterize cellular subtypes and cognate genetic markers using single nuclear RNA sequencing on 8 NMIBC samples treated with BCG, including 3 cases exhibited disease progression. Overall design: 14 samples from 9 patients with BCG. 3 cases show progression after BCG treatment; NP = non-progression, P = Progression sample, CF = Cancer Cell Free

本研究旨在通过对8例经卡介苗（Bacillus Calmette-Guérin, BCG）治疗的非肌层浸润性膀胱癌（Non-Muscle Invasive Bladder Cancer, NMIBC）样本开展单细胞核RNA测序，以解析细胞亚型及其同源遗传标记，其中3例样本出现疾病进展。
总体设计：共纳入9名接受卡介苗治疗的患者的14份样本，其中3例在卡介苗治疗后出现疾病进展；NP代表无进展样本，P代表进展样本，CF代表游离癌细胞（Cancer Cell Free）样本。