High throughput phenotypic screen and transcriptional analysis identify new compounds, targets and pathways for macrophage reprogramming I

Purpose: To understand compound effects on human macrophage polarization in the transcriptional level Fresh isolated human blood monocytes were cultured with 50ng/mL M-CSF for 7 days to generate M0 status macrohages. Macrophages were treated with different chemicals or cytokines for 24 hrs. RNA was isolated and purified by Qiagen Rneasy micro kit and applied to RNAseq.

研究目的：探究化合物对人巨噬细胞极化（macrophage polarization）的转录水平复合效应。将新鲜分离的人外周血单核细胞以50ng/mL巨噬细胞集落刺激因子（M-CSF）培养7天，诱导生成M0型巨噬细胞。将所得巨噬细胞与不同化学物质或细胞因子共处理24小时。采用Qiagen RNeasy 微量试剂盒（Qiagen RNeasy Micro Kit）提取并纯化总RNA，随后进行RNA测序（RNAseq）。