Salicaceae endophyte inoculation alters stomatal patterning and improves the intrinsic water-use efficiency of Populus trichocarpa after a water-deficit

Microorganisms may enhance plant resilience to water stress by influencing their hosts’ physiology and anatomy at the leaf-level. Bacterial and yeast endophytes, isolated from wild poplar and willow, can improve the intrinsic water-use efficiency (iWUE) of cultivated poplar (Populus) under water-deficits by lowering stomatal conductance (gsw). However, the relevance of stomatal anatomy underlying this reduction remains unclear. We hypothesized endophyte inoculation could change host stomatal anatomy, and this would relate to decreases in gsw. We subjected Salicaceae endophyte-inoculated and uninoculated Populus trichocarpa to well-watered and water-deficit treatments in greenhouse studies. We examined the changes of individual stomatal traits and related the composition of these parameters, termed stomatal patterning, to leaf gas-exchange under light saturation. After a water-deficit, inoculation improved iWUE at light saturation from preserving carbon assimilation (Anet) and lowering g..., , , # Data from: Salicaceae endophyte inoculation alters stomatal patterning and improves the intrinsic water-use efficiency of Populus trichocarpa after a water-deficit

[https://doi.org/10.5061/dryad.p5hqbzkz3](https://doi.org/10.5061/dryad.p5hqbzkz3)

## Description of the data and file structure

Microorganisms may enhance plant resilience to water stress by influencing their hosts’ physiology and anatomy at the leaf-level. Bacterial and yeast endophytes, isolated from wild poplar and willow, can improve the intrinsic water-use efficiency (iWUE) of cultivated poplar (Populus) under water-deficits by lowering stomatal conductance (gsw). However, the relevance of stomatal anatomy underlying this reduction remains unclear. We hypothesized endophyte inoculation could change host stomatal anatomy, and this would relate to decreases in gsw. We subjected Salicaceae endophyte-inoculated and uninoculated Populus trichocarpa to well-watered and water-deficit treatments in greenhouse studies. We e...

微生物可通过在叶片层面调控宿主的生理与解剖结构，提升植物对水分胁迫的耐受性。分离自野生杨和柳的细菌与酵母内生菌，能通过降低气孔导度（gsw），改善水分亏缺条件下栽培杨（Populus）的内在水分利用效率（iWUE）。然而，这一gsw降低现象背后的气孔解剖学机制相关性仍不明确。我们假设内生菌接种可改变宿主气孔解剖结构，且该变化与gsw降低存在关联。在温室试验中，我们对经杨柳科内生菌接种与未接种的毛果杨（Populus trichocarpa）施加充分浇水与水分亏缺处理，检测单个气孔性状的变化，并将这些参数的组合（称为气孔模式）与光饱和条件下的叶片气体交换进行关联分析。水分亏缺后，接种处理通过维持碳同化（Anet）与降低g...，提升了光饱和条件下的iWUE。
# 数据来源：杨柳科内生菌接种改变气孔模式并提升水分亏缺后毛果杨的内在水分利用效率
[https://doi.org/10.5061/dryad.p5hqbzkz3]

## 数据与文件结构描述
微生物可通过在叶片层面调控宿主的生理与解剖结构，提升植物对水分胁迫的耐受性。分离自野生杨和柳的细菌与酵母内生菌，能通过降低气孔导度（gsw），改善水分亏缺条件下栽培杨（Populus）的内在水分利用效率（iWUE）。然而，这一gsw降低现象背后的气孔解剖学机制相关性仍不明确。我们假设内生菌接种可改变宿主气孔解剖结构，且该变化与gsw降低存在关联。在温室试验中，我们对经杨柳科内生菌接种与未接种的毛果杨（Populus trichocarpa）施加充分浇水与水分亏缺处理，我们