Comparison of gene expression from thymocyte populations and equivalent OP9-DL1 cultured cells. Homo sapiens

Comparison between ex vivo immature, mature and stimulated T cells and in vitro generated counterparts. The T cells generated in vitro were cultured on OP9-DL1 stroma supplied with growth factors. Overall design: Three in vivo (Thymus DPCD3+, Thymus SP8, PBMC CD8 POST) and three in vitro (CCd14 DPCD3+, CCd20 SP8, CCd27 Post) samples were collected. T-cells were sorted to high purity, RNA was extracted and hybridized on Affymetrix microarrays. No technical replicates were used. The expression profiles of in vivo samples were then compared to the ones of in vitro samples.

本研究开展离体（ex vivo）未成熟、成熟及活化T细胞与体外（in vitro）诱导生成的对应细胞群的比较分析。本研究中体外诱导生成的T细胞，在添加生长因子的OP9-DL1基质细胞共培养体系中完成培养。实验整体设计：共收集3组体内（in vivo）来源样本（胸腺DPCD3+、胸腺SP8、PBMC CD8 POST）与3组体外来源样本（CCd14 DPCD3+、CCd20 SP8、CCd27 Post）。对T细胞进行高纯度分选，提取总RNA后在Affymetrix微阵列芯片上进行杂交。本实验未设置技术重复样本，随后对体内来源样本与体外来源样本的基因表达谱开展对比分析。