Additional file 13: of A positive feed-forward loop between LncRNA-URRCC and EGFL7/P-AKT/FOXO3 signaling promotes proliferation and metastasis of clear cell renal cell carcinoma

Figure S4. A: Bioinformatics analysis of potential FOXO3 binding site on URRCC promoter by online software RegRNA 2.0. B: Correlation between URRCC and FOXO3 in mRNA level in cell lines. C and D: The mRNA level of URRCC and EGFL7 were detected by qRT-PCR in A498 and OSRC-2 cells after transfection with si-NC or si-FOXO3. E and F: The mRNA level of URRCC and EGFL7 were detected by qRT-PCR in A498 and OSRC-2 cells after transfection with NC or oe-FOXO3. G and H: Cell apoptosis assays by flow cytometry in A498 cell lines. (ZIP 209 kb)

补充图S4。A：通过在线软件RegRNA 2.0对URRCC启动子上的潜在FOXO3结合位点开展生物信息学分析。B：细胞系中URRCC与FOXO3的mRNA水平相关性分析。C、D：在转染阴性对照小干扰RNA（si-NC）或FOXO3靶向小干扰RNA（si-FOXO3）的A498及OSRC-2细胞中，采用实时荧光定量逆转录聚合酶链反应（qRT-PCR）检测URRCC与EGFL7的mRNA表达水平。E、F：在转染阴性对照（NC）或FOXO3过表达载体（oe-FOXO3）的A498及OSRC-2细胞中，采用qRT-PCR检测URRCC与EGFL7的mRNA表达水平。G、H：采用流式细胞术对A498细胞系进行细胞凋亡检测实验。(ZIP 209 KB)