IFNgR1 staining in neural cells

Neonatal and adult mouse brains were analyzed for expression of the gamma-interferon receptor subunit R1. Hippocampal and cerebellar samples were assayed for IFNgR1 expression on mature neurons (BIII-tubulin+), immature neurons (DCX+), neural stem cells (Nestin+), astrocytes (GFAP+), and macrophage/microglia (CD45+/CD11b+). Panel A included the following antibodies: anti-IFNGR1-488, anti-BIII tubulin-PE, and anti-nestin-AF647. Panel B included the following antibodies: IFNgR1– AF488 and GFAP – PE. Panel C included the following antibodies: IFNgR1– AF488, DCX– PE, CD45 – PerCP, and CD11b– APC. Fluoresence minus one (FMO) staining controls were used. Unstained and Fluorescence minus one (FMO) staining controls were used.

本研究对新生及成年小鼠脑组织进行了γ-干扰素受体亚基R1（gamma-interferon receptor subunit R1）的表达分析。研究人员对海马体与小脑样本进行检测，分析了成熟神经元（BIII微管蛋白阳性，即BIII-tubulin+）、未成熟神经元（双皮质素阳性，即DCX+）、神经干细胞（巢蛋白阳性，即Nestin+）、星形胶质细胞（胶质纤维酸性蛋白阳性，即GFAP+）以及巨噬细胞/小胶质细胞（CD45+/CD11b+）表面的IFNgR1表达水平。
图版A所用抗体包括：抗IFNgR1-488、抗BIII微管蛋白-PE以及抗巢蛋白-AF647。
图版B所用抗体包括：抗IFNgR1-AF488与抗GFAP-PE。
图版C所用抗体包括：抗IFNgR1-AF488、抗双皮质素（DCX）-PE、抗CD45-PerCP以及抗CD11b-APC。
本实验使用了未染色对照与荧光减一（Fluorescence minus one, FMO）染色对照，该对照设置在原文中被重复提及。