Genomic occupancy of the INO80 complex [II]

We mapped 4 subunits of the INO80 complex using ChIP-seq in HepG2 liver cancer cells. We found a subclass of sites occupied by the INO80 ATPase subunit, but not by any accessory subunits that we call 'Non-canonical' INO80 sites. These sites are characterized by repressed chromatin. ChIP-seq was performed for INO80 subunits in HepG2 cells and was processed for ChIP as previously described (Raab et al 2015), followed by high throughput sequencing. Reanalysis of GSE97411 Samples.

本研究在HepG2肝癌细胞中通过染色质免疫共沉淀测序（ChIP-seq）绘制了INO80复合物（INO80 complex）4个亚基的染色质结合图谱。我们发现了一类仅被INO80 ATP酶亚基（ATPase subunit）占据、却未被任何辅助亚基结合的位点，我们将其命名为"非经典"INO80位点（Non-canonical INO80 sites）。此类位点以染色质抑制为特征。针对HepG2细胞中INO80亚基的ChIP-seq实验，其染色质免疫共沉淀步骤参照此前发表的方法（Raab等，2015）完成，后续开展了高通量测序（high-throughput sequencing）。本研究同时对GSE97411数据集的样本进行了重新分析。