Connecting cellular water behaviour to drug action

Considering that the dynamical window covered by IRIS (or OSIRIS) is within the observed diffusion coefficient range for intracellular water, this proposal aims to achieve a much better understanding of the behaviour of cellular water before and after being treated by the monoclonal antibody tocilizumab (TCZ); either free of, or encapsulated in, a newly developed biopolymeric nanoparticle. It is expected that cells treated with free or encapsulated TCZ, as opposed to cells treated only with the NPs, will present a different flexibility when compared to untreated cells (control).

鉴于IRIS（或OSIRIS）所覆盖的动态窗口处于细胞内水的观测扩散系数（diffusion coefficient）范围内，本研究旨在深入理解细胞水在经单克隆抗体托珠单抗（tocilizumab，TCZ）处理前后的行为特征——无论是游离态的TCZ，还是包裹于新型生物聚合物纳米颗粒（biopolymeric nanoparticle）中的TCZ。预期经游离或包裹态TCZ处理的细胞，与仅经纳米颗粒（NPs）处理的细胞相比，其柔性将与未处理细胞（对照组）存在差异。