Translational Reprogramming as a Driver of Antimony-drug Resistance in Leishmania

Two types of changes were examined at the translatome level in Leishmania: changes at the translational level in drug-resistant strain occurring in the absence of the drug (general basal changes), and those only detectable when the resistant parasites are growing under SbIII exposure (changes to combat the drug). Supplementary files description: File name: Supplementary Data 1 Description: Raw data of automatic polysome fractionation per experimental condition. File name: Supplementary Data 2: Description: Differentially expressed genes detected in antimony-resistant parasites at the basal level (no drug challenge). File name: Supplementary Data 3: Description: Raw data from Venn diagram summarizing the differentially expressed genes detected at the basal level (no drug challenge) per polysome fraction. File name: Supplementary Data 4: Description: Comparison of the translatome Vs. transcriptome (basal level). File name: Supplementary Data 5: Description: Dataset of differentially expressed genes detected in antimony resistant parasites growing under drug challenge. File name: Supplementary Data 6: Description: Raw data from Venn diagram summarizing the differentially expressed genes detected in antimony resistant parasites growing under drug challenge per polysome fraction. File name: Supplementary Data 7: Description: Comparison of the translatome Vs. transcriptome (drug challenge). File name: Supplementary Data 8: Description: Dataset summarizing the normalized read counts by DESeq2’s median of ratios. File name: Supplementary Data 9: Description: List of primers used for the validation of translatome analysis by RT-qPCR. File name: Supplementary Data 10: Description: Comparison between translatomic and proteomic analyses for resistant parasites growing under drug challenge. File name: Supplementary Data 11: Description: Dataset summarizing known and potentially new antimony modulators File name: SupplementaryData 12: Description: Raw data of gene ontology enrichment analysis. File name: Supplementary Data 13: Description: Clustering analysis based on protein homology summarizing changes at the basal level (no drug challenge). File name: Supplementary Data 14: Description: Clustering analysis based on protein co-expression summarizing changes at the basal level (no drug challenge). File name: Supplementary Data 15: Description: Raw data for the glutathione metabolism STRING network dataset summarizing changes under drug challenge. File name: Supplementary Data 16: Description: Manually grouped differentially translated transcripts detected in antimony resistant parasites growing under drug challenge. Supplementary Data 17: Description: Gene variants detected in antimony resistant parasites by using the FreeBayes algorithm. Supplementary Data 18: Description: Comparison between translatomic and variant calling analyses.

本研究针对利什曼原虫（Leishmania）的翻译组（translatome）层面展开两类变化的考察：其一为耐药菌株在无药物暴露条件下发生的翻译水平改变（即基础普遍性变化）；其二为仅当耐药寄生虫于三价锑（SbIII）暴露环境中增殖时方可被检测到的变化（即药物应对性变化）。

补充文件说明如下：
1. 文件名：Supplementary Data 1
描述：各实验条件下自动多聚核糖体分级分离的原始数据
2. 文件名：Supplementary Data 2
描述：基础状态（无药物刺激）下锑剂耐药寄生虫中检测到的差异表达基因数据集
3. 文件名：Supplementary Data 3
描述：按多聚核糖体分级汇总基础状态（无药物刺激）下差异表达基因的韦恩图原始数据
4. 文件名：Supplementary Data 4
描述：翻译组与转录组（基础状态）的对比分析数据
5. 文件名：Supplementary Data 5
描述：药物暴露条件下增殖的锑剂耐药寄生虫中检测到的差异表达基因数据集
6. 文件名：Supplementary Data 6
描述：按多聚核糖体分级汇总药物暴露条件下增殖的锑剂耐药寄生虫差异表达基因的韦恩图原始数据
7. 文件名：Supplementary Data 7
描述：翻译组与转录组（药物刺激条件）的对比分析数据
8. 文件名：Supplementary Data 8
描述：经DESeq2中位数比率法归一化的读段计数汇总数据集
9. 文件名：Supplementary Data 9
描述：用于通过逆转录实时定量PCR（RT-qPCR）验证翻译组分析的引物列表
10. 文件名：Supplementary Data 10
描述：药物暴露条件下增殖的耐药寄生虫的翻译组与蛋白质组分析对比数据
11. 文件名：Supplementary Data 11
描述：已知及潜在新型锑剂调控因子汇总数据集
12. 文件名：Supplementary Data 12
描述：基因本体（Gene Ontology）富集分析原始数据
13. 文件名：Supplementary Data 13
描述：基于蛋白质同源性的聚类分析数据，汇总基础状态（无药物刺激）下的变化情况
14. 文件名：Supplementary Data 14
描述：基于蛋白质共表达的聚类分析数据，汇总基础状态（无药物刺激）下的变化情况
15. 文件名：Supplementary Data 15
描述：药物暴露条件下谷胱甘肽代谢的STRING相互作用网络数据集原始数据
16. 文件名：Supplementary Data 16
描述：药物暴露条件下增殖的锑剂耐药寄生虫中经手动分组的差异翻译转录本数据集
17. 文件名：Supplementary Data 17
描述：通过FreeBayes算法检测到的锑剂耐药寄生虫基因变异数据集
18. 文件名：Supplementary Data 18
描述：翻译组分析与变异检出分析的对比数据