Raw Dataset for "Emerging dactylellinatone attractant aids in the predation of nematodes by nematode-trapping fungus Dactylellina haptotyla"

We identified dactylellinatone, a novel nematode chemoattractant from <i>D. haptotyla</i> that enhances fungal predatory efficiency. Heterologous expression and isotope-labeled feeding experiments confirmed that the <i>Dlt</i> gene cluster is directly responsible for the biosynthesis of dactylellinatone. We discovered that the expression of <i>Dlt</i> gene cluster was significantly upregulated during predation. Deletion of the core gene in the cluster not only disrupted dactylellinatone production but also reduced <i>D. haptotyla</i>'s attraction to nematodes and its pathogenicity. Collectively, our work reveals that <i>D. haptotyla</i> can increase their predation efficiency when sensing the presence of nematodes by triggering the expression of <i>Dlt </i>gene cluster and initiating the synthesis of dactylellinatone.

我们从捕食线虫真菌Dactylellina haptotyla（缩写为D. haptotyla）中鉴定出一种新型线虫化学引诱剂dactylellinatone，该物质可提升真菌的捕食效率。通过异源表达实验与同位素标记饲喂实验，我们证实Dlt基因簇（Dlt gene cluster）直接负责dactylellinatone的生物合成。我们发现，在捕食过程中，Dlt基因簇的表达量显著上调。敲除该基因簇中的核心基因，不仅会阻断dactylellinatone的合成，还会削弱D. haptotyla对线虫的引诱能力及其致病性。综上，本研究揭示，D. haptotyla可通过感知线虫的存在，激活Dlt基因簇的表达并启动dactylellinatone的合成，进而提升自身的捕食效率。