Additional file 2 of Mesothelioma-associated fibroblasts enhance proliferation and migration of pleural mesothelioma cells via c-Met/PI3K and WNT signaling but do not protect against cisplatin

Additional file 2: Supplementary Table S1. Overview of cell types used in the study. Supplementary Table S2. List of primers used for qRT-PCR and evaluation of germline DNA mutations. Supplementary Table S3. Genes investigated by next generation sequencing in the TruSight Oncology 500 panel. Supplementary Table S4. Differentially expressed genes of Meso-CAFs compared to PM cells. Supplementary Table S5. Genes with high fold change in Meso-CAFs compared to CAF-3. Supplementary Table S6. Numbers of identified proteins in the nuclear, cytoplasmic and supernatant fractions of Meso-CAFs and NLFs. Supplementary Table S7. Identified proteins of Meso-CAFs in the nuclear, cytoplasmic and supernatant fraction. Supplementary Table S8. Identified proteins of NLFs in the nuclear, cytoplasmic and supernatant fraction. Supplementary Table S9. Actively secreted proteins exclusively identified in Meso-CAFs or significantly upregulated compared to NLFs.

附加文件2：补充表S1 本研究所使用的细胞类型概述。补充表S2 用于定量实时聚合酶链式反应（qRT-PCR）及生殖系DNA突变评估的引物列表。补充表S3 TruSight Oncology 500测序面板中经下一代测序（next generation sequencing）检测的基因。补充表S4 间皮相关成纤维细胞（Meso-CAFs）相较于PM细胞的差异表达基因。补充表S5 相较于CAF-3，间皮相关成纤维细胞（Meso-CAFs）中表达倍数差异显著的基因。补充表S6 间皮相关成纤维细胞（Meso-CAFs）与正常肺成纤维细胞（NLFs）的细胞核、细胞质及细胞上清液组分中鉴定到的蛋白质数量。补充表S7 间皮相关成纤维细胞（Meso-CAFs）的细胞核、细胞质及细胞上清液组分中鉴定到的蛋白质。补充表S8 正常肺成纤维细胞（NLFs）的细胞核、细胞质及细胞上清液组分中鉴定到的蛋白质。补充表S9 仅在间皮相关成纤维细胞（Meso-CAFs）中鉴定到，或相较于正常肺成纤维细胞（NLFs）显著上调的主动分泌蛋白。