Pseudomonas mandelii 36MFCvi1.1. Pseudomonas mandelii 36MFCvi1.1

Rhizosphere Grand Challenge Isolate Sequencing. The goal of this project is to comprehensively characterize the genomes of microorganisms found in the rhizosphere and endosphere of Arabidopsis thaliana. Organisms for study have been targeted based on their reproducible association with one or more Arabidopsis genotypes grown in natural soil. To obtain more complete genomes from the culturable fraction of the Arabidopsis microbiome, rhizosphere and endosphere samples were used to seed cultures on a variety of media. Cultures were screened by 16S rRNA sequencing and microbes of interest grown up and subjected to whole genome sequencing. Genomic sequences will be analyzed for genes participating in the plant-microbe symbiosis.

根际重大挑战分离株测序（Rhizosphere Grand Challenge Isolate Sequencing）。本项目的目标是全面解析拟南芥（Arabidopsis thaliana）根际（rhizosphere）与内生境（endosphere）中微生物的基因组特征。本研究所选用的微生物菌株，均依据其与自然土壤中栽培的一种或多种拟南芥基因型可重复的共生关联特征完成筛选。为从拟南芥微生物组的可培养组分中获取更为完整的基因组序列，研究人员以根际与内生境样本为接种源，在多种培养基上开展菌株培养。通过16S rRNA测序对培养物进行筛选，获得目标微生物后开展全基因组测序。后续将对获取的基因组序列进行分析，以鉴定参与植物-微生物共生过程的功能基因。