Sex differences in the developmental patterns of the cortical astroglial cell translatome

Employing translating ribosome affinity purification with RNA sequencing (TRAPseq) on cortical tissue to isolate astroglial-specific translatome from Aldh1l1-L10-GFP mice and examine sex (male + female) and age (postnatal days 1, 4, 7, 14, 35; adults (9 weeks)) dependent changes in the astroglial translatome. Overall design: Cortical lysate (N=53 independent samples) from male and female Aldh1l1-GFP reporter mice (C57BL/6 background strain) at 6 different postnatal time points was used for translating ribosome affinity purification (TRAP) to extract actively transcribed RNA and then used with Illumina Hi-Seq RNA sequencing. Four of the samples (P7 Female 10, P7 Female 6, P7 Male 2, P7 Male 6) were sequenced twice.

本研究采用翻译核糖体亲和纯化结合RNA测序（TRAPseq）技术，对Aldh1l1-L10-GFP小鼠的皮层组织开展实验，以分离星形胶质细胞特异性翻译组，并探究性别（雄性与雌性）及年龄（出生后第1、4、7、14、35天；成年小鼠为9周龄）相关的星形胶质细胞翻译组动态变化。整体实验设计：收集6个不同出生后时间点的雄性与雌性Aldh1l1-GFP报告基因小鼠（C57BL/6背景品系）的皮层裂解物，合计53份独立样本。将上述样本用于翻译核糖体亲和纯化（TRAP）以提取活跃转录的RNA，随后通过Illumina Hi-Seq RNA测序进行分析。其中4份样本（P7雌性10号、P7雌性6号、P7雄性2号、P7雄性6号）进行了两次测序。