小鼠胚胎干细胞ES-D3 [D3]
收藏中国科技资源共享网2026-06-09 更新2026-01-30 收录
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https://escience.org.cn/metadata/detail?cstrId=CSTR:19375.09.3101MOUSCSP202&id=72176a45901492835836654ac2eaf714:3101MOUSCSP202
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资源简介：
在没有饲养层或条件培养基的情况下，ES-D3 [D3]细胞会自发分化为胚胎结构。可以将它们注射回囊胚并能进入生殖系。未分化的D3细胞可以通过基因靶向技术进行遗传修饰。每个批次均通过本库支原体检测，结果为阴性。培养时需使用昆明白MEF（SCSP-101R）作为饲养层细胞。冻存管细胞为P12。其重要性在于可为生命科学和生物技术领域的研究工作提供标准化的实验细胞资源及相关技术支撑。
ES-D3 [D3] cells spontaneously differentiate into embryonic structures in the absence of feeder layers or conditioned medium. They can be injected back into blastocysts and contribute to the germline. Undifferentiated D3 cells can be genetically modified via gene targeting techniques. Each batch has been tested negative for mycoplasma by this cell bank. Kunming white MEF (SCSP-101R) should be used as feeder cells during culture. The cells in the cryopreserved vials are at passage 12 (P12). This cell resource provides standardized experimental cell resources and relevant technical support for research in the fields of life sciences and biotechnology, holding significant research value.
提供机构：
国家模式与特色实验细胞资源库
创建时间：
2021-06-18
