Global transcriptomic analysis of iPSC-derived hepatocytes infected with Ebola virus (EBOV). Global transcriptomic analysis of iPSC-derived hepatocytes infected with Ebola virus (EBOV)

We performed transcriptomic analysis of a time course of Ebola virus (EBOV) iPSC-derived hepatocytes. Mock infected and EBOV infected hepatocytes were harvested 1, 2, 3, and 7 days post-infection for analysis. Hepatocytes were infected with EBOV at a multiplicity of infection of 10. Overall design: Global transcriptomic analysis of iPSC-derived hepatocytes infected with Ebola virus (EBOV) and harvested at 1, 2, 3, and 7 days post-infection. Corresponding, uninfected cells were harvested at matching time points. Duplicate samples (n=2) were submitted for analysis.

我们针对埃博拉病毒（Ebola virus, EBOV）感染诱导多能干细胞（induced pluripotent stem cell, iPSC）衍生肝细胞的时间进程开展了转录组分析。分别于感染后1、2、3及7天，收集模拟感染与埃博拉病毒感染的肝细胞样本用于分析。本研究以感染复数（multiplicity of infection, MOI）10的剂量感染肝细胞。实验设计概述：对埃博拉病毒感染的诱导多能干细胞衍生肝细胞开展全局转录组分析，分别于感染后1、2、3及7天收集样本；同时在匹配的时间点收集未感染的对照细胞。所有样本均设置2个生物学重复（n=2）并提交分析。