The trajectories of pro-neural phenotype switch induced by NGN2, ASCL1, SOX2, and MSI1 in human fibroblasts

Direct pro-neural reprogramming is a conversion of differentiated somatic cells toneural cells without an intermediate pluripotency stage. It is usually achieved via an ectopicexpression (EE) of certain transcriptional factors (TFs) or other reprogramming factors (RFs).Determining transcriptional changes (TCs) caused by particular RFs in a given cell line enables aninformed approach to reprogramming initiation. Here, we characterized TCs in the humanfibroblast cell line LF1 at 5th day after EE of single well-known pro-neuronal RFs NGN2, ASCL1,SOX2, and MSI1.

直接促神经元重编程（direct pro-neural reprogramming）是指将已分化的体细胞无需经过中间多能性阶段，直接转化为神经细胞的过程。该过程通常通过特定转录因子（transcriptional factors, TFs）或其他重编程因子（reprogramming factors, RFs）的异位表达（ectopic expression, EE）实现。明确特定重编程因子在指定细胞系中引发的转录变化（transcriptional changes, TCs），可为重编程启动提供科学有据的研究思路。本研究对人成纤维细胞系LF1在单个经典促神经元重编程因子NGN2、ASCL1、SOX2及MSI1异位表达后的第5天的转录变化进行了表征。