Transcriptional response of springtail (Folsomia candida) exposed to decabromodiphenyl ether-contaminated soil. Transcriptional response of springtail (Folsomia candida) exposed to decabromodiphenyl ether-contaminated soil

To assess the effects of decabromodiphenyl ether (BDE209) on gene transcription of Folsomia candida and explore the mechanisms of toxicity, we detected changes in gene transcription of F. candida exposed to BDE209 (0.81 mg/kg) in soil for 2, 7 and 14 days. We identified 16 and 771 significantly differentially expressed genes after 2 and 7 days of exposure respectively, and no significantly regulated genes were shared among the two time points. No genes were affected after 14 days of exposure. Our results suggeted that BDE209 affected the transcription of genes involved in moulting, neural signal transmission and detoxification. Overall design: Thirty adult springtails (23-day old) were exposed to BDE209-polluted soil or solvent control soil in 100 mL glass beakers for 2, 7 or 14 days.

为评估十溴二苯醚（decabromodiphenyl ether, BDE209）对弹尾虫（Folsomia candida）基因转录的影响并探索其毒性作用机制，本研究检测了暴露于含0.81 mg/kg BDE209土壤中的弹尾虫在暴露2、7和14天后的基因转录变化。研究分别在暴露2天和7天后鉴定出16个和771个显著差异表达基因，且两个时间点无共有显著调控基因；暴露14天后未检测到受影响的基因。本研究结果表明，BDE209可影响与蜕皮、神经信号传递及解毒作用相关的基因转录。
实验设计：将30只成年弹尾虫（23日龄）置于100 mL玻璃烧杯中，暴露于BDE209污染土壤或溶剂对照土壤中，分别培养2、7或14天。