Patterns of cancer development and progression in the protein-protein interaction network

For this study we selected a gene, α-synuclein (SNCA), that is consistently under-expressed in MCF7 cells and breast tumors. Following transfection with an SNCA expression construct, two stable MCF7 clones (named MCF7-SNCA #1 and 2) were selected and examined for expression differences relative to the parental MCF7 cells. MCF7 cells were transfected with an expression vector containing the full-length SNCA sequence. After selection with blasticidin, two different clones were isolated and examined for expression differences relative to the parental MCF7 cells.

本研究选取α-突触核蛋白（α-synuclein, SNCA）作为研究对象，该基因在MCF7细胞与乳腺肿瘤中均呈持续低表达状态。将SNCA表达构建体转染MCF7细胞后，我们筛选得到两株稳定MCF7克隆（命名为MCF7-SNCA #1和#2），并检测其与亲本MCF7细胞的表达差异。我们将携带全长SNCA序列的表达载体转染MCF7细胞，经杀稻瘟菌素（blasticidin）筛选后，分离得到两株不同的克隆，并检测其与亲本MCF7细胞的表达差异。