Expression data in Arabidopsis wild type, ahg2-1 and ahg2-1ags1-1 mutants

We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis wild type, ahg2-1 and ahg2-1ags1-1 mutants. The total RNA was prepared from 15-day-old Arabidopsis plants grown on the agar plates and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

本研究采用平铺式微阵列（tiling array）技术，对野生型拟南芥、ahg2-1突变体及ahg2-1ags1-1突变体的全基因组转录组展开分析。实验材料取自生长于琼脂平板上的15日龄拟南芥植株，提取其总RNA后用于微阵列杂交。针对每条链阵列，我们使用独立的生物学重复RNA样本完成了三次重复杂交实验。