Additional file 2 of Stage-specific transcriptomic changes in pancreatic α-cells after massive β-cell loss

Additional file 2: Supplementary Table 1. Alignment details for raw RNA-seq and ChIP-seq data used in this study. Supplementary Table 2. Gene Set Enrichment Analysis significant (p<0.05, FDR<0.25) results obtained from pairwise sample comparisons. Categories mentioned in the manuscript are highlighted in bold. Supplementary Table 3. Genes excluded from the final clustering analysis. Supplementary Table 4. Lists of “α-cell transitional genes” including gene cluster association capturing the main differences between α-cells at different stages after β-cell loss. Number of human pancreatic islet enhancers, and number islet transcription binding sites for five islet transcription factors, that were associated with the promoters of genes in clusters 1 to 5. Supplementary Table 5. Statistical tests for genes showed in Figure 4B. Supplementary Table 6. Number of cells counted for the quantifications presented in the manuscript figures.

附加文件2：
补充表1：本研究所用原始RNA测序（RNA-seq）与染色质免疫沉淀测序（ChIP-seq）数据的比对详情。
补充表2：经成对样本比较得到的具有统计学显著性（p<0.05，错误发现率（False Discovery Rate, FDR）<0.25）的基因集富集分析（Gene Set Enrichment Analysis, GSEA）结果，论文中提及的分类已以粗体标注。
补充表3：最终聚类分析中剔除的基因列表。
补充表4：“α细胞过渡基因”列表，包含可体现β细胞丢失后不同阶段α细胞核心差异的基因聚类关联信息；以及与聚类1至5中基因启动子相关的人类胰岛增强子数量和5种胰岛转录因子的胰岛转录结合位点数量。
补充表5：图4B中展示基因的统计学检验结果。
补充表6：论文各配图中量化分析所用的计数细胞数量。