Severe acute respiratory syndrome coronavirus 2 Heterologous Re-challenge Genome sequencing and assembly

Here, we deep sequenced multiple BAL samples of SARS-CoV-2 from Non-human primates that were re-challenged with BIOQUAL's RSA-030621-750 stock (B.1.351). The vRNA of these stocks were reverse-transcribed in duplicate using the ARTIC protocol and prepped to be sequenced on a Illumina MiSeq by using a Truseq Library prep kit.

本研究对经BIOQUAL公司RSA-030621-750毒种（B.1.351株）再次攻毒的非人灵长类动物的多份支气管肺泡灌洗液（Bronchoalveolar Lavage, BAL）样本中的严重急性呼吸综合征冠状病毒2（Severe Acute Respiratory Syndrome Coronavirus 2, SARS-CoV-2）开展了深度测序。该毒种的病毒RNA（viral RNA, vRNA）采用ARTIC实验方案（ARTIC protocol）进行双份平行逆转录，随后使用Truseq文库制备试剂盒（Truseq Library Prep Kit）构建测序文库，并在因美纳（Illumina）MiSeq测序平台上完成上机测序。