Microbial Dynamics in Surti Buffalo Dung and Urine-Based Fermented Plant Growth Promoting Formulation

In this study, we developed a plant growth-promoting formulation using Surti buffalo (a breed of water buffalo, Bubalus bubalis), dung and urine, combined with jaggery, gram flour, soil, and water. The mixture was allowed to ferment. Whole genome sequencing (WGS) metagenomic analysis was conducted on samples taken before fermentation (Day 0) and after fermentation (Day 8). The initial microbial community at Day 0 primarily consisted of diverse bacterial populations inherent to buffalo dung and soil. By Day 8, significant shifts in the microbial composition were observed, indicating the proliferation of beneficial microorganisms facilitated by the fermentation process. This transformation underscores the potential of such bioformulations in enhancing soil fertility and promoting sustainable agricultural practices.

本研究以苏蒂水牛（Surti buffalo，水牛种（Bubalus bubalis））的粪便与尿液为原料，搭配粗蔗糖、鹰嘴豆粉、土壤与水，开发了一款植物生长促生制剂。将上述混合物料置于发酵体系中进行发酵。分别采集发酵前（第0天）与发酵后（第8天）的样本，开展全基因组测序（WGS）宏基因组分析。发酵初始阶段（第0天）的微生物群落主要由水牛粪便与土壤中固有的多样化细菌类群构成。至发酵第8天时，微生物群落组成出现显著变化，表明发酵过程推动了有益微生物的增殖。这一转化过程凸显了此类生物制剂在提升土壤肥力、促进可持续农业实践方面的应用潜力。