Evaluation of the physiological property of D-allulose on obesity and its comorbidities in diet-induced obese mice based on mRNA-seq analysis

ackground/Objectives: The aim of the current study was to elucidate the effects of long-term supplementation with D-allulose on obesity and associated comorbidities by analyzing transcriptional and metabolic responses. Subjects/Methods: C57BL/6J mice were divided into three groups and fed a normal diet, high-fat diet (HFD), or high-fat + 5% (w/w) D-allulose diet for 16 weeks. Results: Body weight and body fat mass were significantly decreased in HFD-fed with D-allulose supplemented mice and their levels were similar to that of the normal diet. Also, major symptoms of obesity, such as high plasma lipid profiles and cytokine levels, were attenuated by D-allulose supplementation. D-allulose supplement induced the alteration of mRNA expression in epididymal white adipose tissue (eWAT) and hepatic tissue. D-allulose normalized mRNA expression related lipid metabolism in eWAT and hepatic tissue. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway mapper analyses, D-allulose supplementation were down regulated the “cytokine-cytokinereceptor interaction”, “chemokinesignaling pathway”, “MAPK signaling pathway”and“toll-like receptor signaling pathway” in eWAT and hepatic tissue. Overall design: Total RNA of liver and adipose tissues was obtained from normal diet, high-fat diet and D-allulose added high-fat diet-fed mice and mRNA expression-associated with lipid metabolism and inflammation was measured.

研究背景与目的：本研究旨在通过分析转录与代谢响应，阐明长期补充D-阿洛酮糖（D-allulose）对肥胖及其相关并发症的影响。
实验对象与方法：将C57BL/6J小鼠分为三组，分别饲喂正常饲料、高脂饲料（HFD）以及高脂+5%（w/w）D-阿洛酮糖饲料，干预周期为16周。
结果：饲喂添加D-阿洛酮糖的高脂饲料的小鼠，其体重与体脂量均显著降低，且相关指标水平与正常饲料组小鼠相近。此外，肥胖的典型表征，如血浆脂质谱异常与细胞因子水平升高，均可通过补充D-阿洛酮糖得到缓解。D-阿洛酮糖可诱导附睾白色脂肪组织（eWAT）与肝组织的信使RNA（mRNA）表达谱发生改变，并可使上述组织中与脂质代谢相关的mRNA表达恢复正常。在京都基因与基因组百科全书（KEGG）通路映射分析中，补充D-阿洛酮糖可下调附睾白色脂肪组织与肝组织中的“细胞因子-细胞因子受体相互作用”“趋化因子信号通路”“丝裂原活化蛋白激酶（MAPK）信号通路”以及“Toll样受体信号通路”。
整体实验设计：提取正常饲料组、高脂饲料组及高脂添加D-阿洛酮糖组小鼠的肝脏与脂肪组织总RNA，检测与脂质代谢及炎症相关的mRNA表达水平。