BMP signaling in mouse embryonic stem cells (ESCs) in the naïve and primed pluripotent states [RNA-Seq]. Mus musculus

Carrying out both RNA-seq and Smad1/5 genome-wide chromatin immunoprecipitation and sequencing (ChIP-seq) analyses of mESCs in the naïve or primed states, we revisit the roles of BMP signaling in mESCs. Overall design: RNA-seq analysis in 2 cell types; mESCs and ES-derived EpiSC (ESD-EpiSCs).

本研究针对处于初始态（naïve）与始发态（primed）的小鼠胚胎干细胞（mESCs），同步开展RNA-seq与Smad1/5全基因组染色质免疫共沉淀测序（ChIP-seq）分析，重新探讨骨形态发生蛋白信号通路（BMP signaling）在小鼠胚胎干细胞中的功能。
实验整体设计：本研究对两类细胞开展RNA-seq分析，分别为小鼠胚胎干细胞以及胚胎干细胞衍生的外胚层干细胞（ES-derived EpiSC, ESD-EpiSCs）。