RNA-seq of Hacat cells treated with TOPK inhibitor

Hacat cells were treated with TOPK inhibitor to elucidate the effect of TOPK signaling on Hacat cells Overall design: Hacat cells were cultured in M5 cocktail, then treated with or without TOPK inhibitor for 24 h. Total RNA were then extracted and subjected to RNA-seq analysis

为阐明TOPK信号通路对Hacat细胞的影响，研究人员使用TOPK抑制剂（TOPK inhibitor）处理Hacat细胞。实验整体设计：将Hacat细胞于M5混合培养液（M5 cocktail）中培养，随后分别施加或不施加TOPK抑制剂处理24小时，之后提取总RNA并进行RNA测序（RNA-seq）分析。