Single-cell transcriptomics of the mouse skin reveal potential target of psoriasis. Single-cell transcriptomics of the mouse skin reveal potential target of psoriasis

Psoriasis is a complex chronic inflammatory skin disease, but the exact mechanism underlying its development remains elusive. We found the expression of SHP2 was significantly higher in skin lesions and human peripheral blood mononuclear cells derived from psoriatic patients than those from normal controls. determine whether SHP2 activity affects psoriasis pathogenesis, we treated the murine psoriatic model with SHP099, a potent allosteric inhibitor of SHP2. SHP099 ameliorates the IMQ-induced psoriatic development in mice with unclear molecular mechanism. To address that, single-cell RNA sequencing was performed to explore the role of SHP2 in all cell types in IMQ-induced mouse skin upon SHP099 treatment. Overall design: Examination of 6 skin samples in IMQ-induced psoriasis-like mouse model

银屑病（Psoriasis）是一种复杂的慢性炎症性皮肤病，但其确切发病机制仍未阐明。本研究发现，银屑病患者皮损及人外周血单个核细胞中SHP2的表达水平显著高于正常对照样本。为明确SHP2活性是否影响银屑病发病机制，我们使用SHP2的强效变构抑制剂SHP099处理银屑病小鼠模型。SHP099可改善小鼠中咪喹莫特（IMQ）诱导的银屑病样病变进程，但其具体分子机制尚未明确。为阐明该机制，本研究通过单细胞RNA测序（single-cell RNA sequencing）探究了SHP2在经SHP099处理的IMQ诱导小鼠皮肤的各类细胞中所发挥的作用。实验设计：对IMQ诱导的银屑病样小鼠模型的6份皮肤样本进行检测。