Gene expression profile with the p38 inhibitor treatment

Mice bone marrow cells induced dendritic cells were treated with a p38 inhibitor, we used Microgenearry to analyze the dendritic cell-related gene expression, which was induced by a p38 inhibitor. Mice were treated with CAR-T cells with or without p38 inhibitor treatment, we used Microgenearry to analyze the dendritic cell-related gene expression in the tumor environment. Tumor tissues (~100 mg/mouse, 12 days after ACT) were harvested and total RNA was extracted with the RNeasy Mini kit (Qiagen). Murine BM cells were cultured and supplemented with mGM-CSF with or without p38 inhibitors. On day 6, semi-adherent cells were collected and total RNA was extracted with the RNeasy Mini kit (Qiagen).

将经p38抑制剂处理的小鼠骨髓源树突状细胞（dendritic cells, DCs）作为研究对象，采用Microgenearry技术分析p38抑制剂诱导的树突状细胞相关基因表达。对经嵌合抗原受体T细胞（chimeric antigen receptor T cells, CAR-T细胞）处理且联合或不联合p38抑制剂干预的小鼠，采用Microgenearry技术分析其肿瘤微环境中的树突状细胞相关基因表达。采集肿瘤组织（每只小鼠约100mg，采集于过继性细胞免疫治疗（adoptive cell transfer, ACT）后第12天），使用RNeasy Mini试剂盒（Qiagen公司）提取总RNA。培养小鼠骨髓细胞，分别添加小鼠粒细胞-巨噬细胞集落刺激因子（mouse granulocyte-macrophage colony-stimulating factor, mGM-CSF）联合或不联合p38抑制剂；于第6天收集半贴壁细胞后，使用RNeasy Mini试剂盒（Qiagen公司）提取总RNA。