Human ECFCs: Cord Blood vs Peripehral Blood ECFCs. Homo sapiens

miRNA profiling was carried out using the miRCURY LNA™ microRNA Array (5th gen - hsa, mmu & rno). microRNA profiling of CB and PB-derived ECFCs from 3 independent donors from each cell source. To identify and understand the regulation of endothelial cell functions through comparing miRNA expression profiling of cord blood (CB) derived endothelial colony forming cells (ECFCs) and peripheral blood (PB) ECFCs Overall design: Two condition experiment, CB vs PB-derived ECFCs. 3 independent donors per ECFC source.

本研究采用miRCURY LNA™ 微小RNA（microRNA）芯片（第5代，覆盖人源（hsa）、小鼠源（mmu）及大鼠源（rno））开展微小RNA表达谱检测。实验样本为脐血（cord blood, CB）来源与外周血（peripheral blood, PB）来源的内皮集落形成细胞（endothelial colony forming cells, ECFCs），每个细胞来源均使用3名独立供体的样本。本实验旨在通过对比两类细胞的微小RNA表达谱，鉴定并解析调控内皮细胞功能的相关分子机制。实验设计为两组对照实验：脐血来源ECFCs与外周血来源ECFCs，每个细胞来源设置3名独立供体作为生物学重复。