Strain identity effects contribute more to Pseudomonas community functioning than strain interactions

Microbial communities can shape key ecological services, but the determinants of their functioning often remain little understood. While traditional research predominantly focuses on effects related to species identity (community composition and species richness), recent work increasingly explores the impact of species interactions on community functioning. Here, we conducted experiments with replicated small communities of fluorescent Pseudomonas bacteria to quantify the relative importance of strain identity versus interaction effects on two important functions, community productivity and siderophore production. By combining supernatant and competition assays with an established linear model method, we show that both factors have significant effects on functioning, but identity effects generally outweigh strain interaction effects. These results hold irrespective of whether strains interactions are inferred statistically or approximated experimentally. Our results have implications fo..., , , # Data from: Strain identity effects contribute more to Pseudomonas community functioning than strain interactions

[https://doi.org/10.1093/ismejo/wraf025](https://doi.org/10.1093/ismejo/wraf025)

The accompanying datafiles contain all the data used in Kramer J, Maréchal S, Figueiredo ART, and Kümmerli R (2024) Strain identity effects contribute more to Pseudomonas community functioning than strain interactions.*The ISME Journal*, 2025; wraf025

## Description of the data and file structure

The four datafiles contain data from supernatant feeding and competition assays with natural *Pseudomonas* bacteria (Kramer_FunctioningDeterminants_Data) as well as sequencing data for phylogenetic analyses of the used strains (Kramer_FunctioningDeterminants_SoilIsolates, Kramer_FunctioningDeterminants_PondIsolates, Kramer_FunctioningDeterminants_Outgroups).

Together with the \"Kramer_FunctioningDeterminants_Rscript\" (see below), this data can be used to replicate the analyses and figures presented...

微生物群落能够塑造关键的生态服务，但影响其功能的决定因素往往仍知之甚少。传统研究主要聚焦于与物种身份相关的效应（群落组成及物种丰富度），而近期研究则日益探索物种间相互作用对群落功能的影响。在此，我们通过荧光假单胞菌（Pseudomonas）的重复小型群落实验，量化了菌株身份与相互作用效应对群落生产力和铁载体（siderophore）产生这两项重要功能的相对重要性。

通过将上清液分析与竞争测定法相结合，并运用成熟的线性模型方法，我们发现这两个因素均对功能具有显著影响，但身份效应通常超过菌株相互作用效应。无论菌株相互作用是通过统计推断还是实验近似获得，这些结果均成立。我们的研究结果对……具有启示意义。

# 数据来源：菌株身份效应对假单胞菌群落功能的贡献大于菌株相互作用
[https://doi.org/10.1093/ismejo/wraf025]

## 数据与文件结构描述

四个数据文件包含天然假单胞菌（Pseudomonas）的上清液喂养及竞争测定数据（Kramer_FunctioningDeterminants_Data），以及所用菌株的系统发育分析测序数据（Kramer_FunctioningDeterminants_SoilIsolates、Kramer_FunctioningDeterminants_PondIsolates、Kramer_FunctioningDeterminants_Outgroups）。结合“Kramer_FunctioningDeterminants_Rscript”（见下文），这些数据可用于复现文中呈现的分析与图表……