Gliding motility assay using 22063mM D2O in solution. Replicate 1/3. ROI 11/15.

• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules.• The buffer used was PEM (also known as BRB80) with the inclusion of 22063mM D2O.• Images were taken with an Andor Luca EMCCD camera on an Olympus IX-71 microscope using a 60x 1.42NA PlanApo objective.• This fileset is replicate 1/3 and ROI 11/15.• Images were taken to investigate speed variations of microtubules due to the inclusion of D2O in the motility assay.• The temperature of the slide was held constant at 32°C by an objective heater. Early ROIs, i.e. 1-5, have slower giding speeds due to the increasing temperature of the slide which, was prepared at room temperature before observation. After ROI 5 the gliding speed stabilizes.• Pixels lengths were measured to be 166nm/pixel using a stage micrometer.• After the files have downloaded, a simple command line entry will convert the images to a movie. Mencoder must be installed. ls > list.txt | mencoder mf://@list.txt -mf w=496:h=658:fps=30:type=png -ovc lavc -lavcopts vcodec=mpeg4:mbd=2:trell -o output.avi• Upload of these files were made possible through the figshare API.

• 本数据集为采用驱动蛋白-1（kinesin-1）与罗丹明标记微管开展的滑行运动分析实验。
• 实验所用缓冲液为PEM（又称BRB80），其中添加了22063mM重水（D₂O）。
• 图像采集使用搭载Andor Luca型电子倍增电荷耦合器件（Electron-Multiplying Charge-Coupled Device，EMCCD）相机的Olympus IX-71型显微镜，搭配60× 1.42NA PlanApo物镜完成拍摄。
• 本文件集对应第1组重复实验（共3组重复）中的第11个感兴趣区域（Region of Interest，ROI，总计15个ROI）。
• 本次图像采集旨在探究滑行运动实验中添加重水对微管运动速度的影响。
• 实验通过物镜加热器将载玻片温度恒定维持在32℃。早期感兴趣区域（即1-5号）的滑行速度较慢，原因在于观测前载玻片于室温下制备，导致其温度随实验推进逐渐升高；5号ROI之后，微管滑行速度趋于稳定。
• 使用载物台测微尺（stage micrometer）校准得到像素标定长度为166nm/像素。
• 文件下载完成后，可通过简单的命令行指令将图像转换为视频。需预先安装Mencoder软件，具体指令为：ls > list.txt | mencoder mf://@list.txt -mf w=496:h=658:fps=30:type=png -ovc lavc -lavcopts vcodec=mpeg4:mbd=2:trell -o output.avi
• 本数据集文件通过figshare API完成上传。