Next Generation Sequencing Facilitates Quantitative Analysis of three groups from mouse liver Transcriptomes

Liver mRNA profiles of WT liver(WT1-WT3) and Alb-Cre:Hdac3-/- liver (0d(Ha-Hc), 7d(Hd-Hg), 21d(Hh-Hk), 50d(Hl-Hn)) receiving successive PH(sPH), Alb-Cre:Hdac3-/- tumor tissues(HCC1-HCC3) and adjacent non-tumor tissues(N1-N3), spontaneous liver cancers of Alb-Cre:Hdac3-/- mice (HCC1-HCC3) and liver tumor of recipient Fah-/- mice (HCCa-HCCc) were generated by deep sequencing. Overall design: mRNA profiles of spontaneous liver cancers of Alb-Cre:Hdac3-/- mice (HCC1-HCC3) and liver tumor of recipient Fah-/- mice (HCCa-HCCc) were used to analyze the Pearson correlation coefficient; liver mRNA profiles of WT liver and Alb-Cre:Hdac3-/- liver (0d(Ha-Hc), 7d(Hd-Hg), 21d(Hh-Hk), 50d(Hl-Hn)) receiving successive PH(sPH), Alb-Cre:Hdac3-/- tumor tissues (HCC1-HCC3) and adjacent non-tumor tissues (N1-N3)were used to analyze the volcano plot, heatmap etc.

本数据集所包含的野生型（Wild Type, WT）肝脏（WT1-WT3）、接受连续部分肝切除术（successive partial hepatectomy, sPH）的Alb-Cre:Hdac3-/-肝脏（0d组Ha-Hc、7d组Hd-Hg、21d组Hh-Hk、50d组Hl-Hn）、Alb-Cre:Hdac3-/-肿瘤组织（HCC1-HCC3）及其配对癌旁非肿瘤组织（N1-N3）、Alb-Cre:Hdac3-/-小鼠自发性肝癌（HCC1-HCC3）以及Fah-/-受体小鼠肝肿瘤（HCCa-HCCc）的mRNA表达谱，均通过深度测序获得。整体实验设计如下：采用Alb-Cre:Hdac3-/-小鼠自发性肝癌（HCC1-HCC3）与Fah-/-受体小鼠肝肿瘤（HCCa-HCCc）的mRNA表达谱进行皮尔逊相关系数分析；采用野生型肝脏、接受连续部分肝切除术的Alb-Cre:Hdac3-/-肝脏（0d组Ha-Hc、7d组Hd-Hg、21d组Hh-Hk、50d组Hl-Hn）、Alb-Cre:Hdac3-/-肿瘤组织（HCC1-HCC3）及癌旁非肿瘤组织（N1-N3）的mRNA表达谱进行火山图、热图等分析。