Data on mitochondrial genome rearrangement patterns, annotation resources, and phylogenetic visualization in Actinopteri (ray-finned fishes)

This dataset integrates structural variations, annotation resources, and phylogenetic analysis results of mitochondrial genomes in ray-finned fishes (Actinopterygii). The raw data were sourced from publicly available mitochondrial genome records in NCBI GenBank (0_gb_actinopteri_14092_from_NCBI.gb), comprising 14,092 original sequences. Standardized annotations were generated using the MITOS online tool, resulting in a compressed package (1_fasta_bed_actinopteri.zip) containing FASTA sequences and BED annotation files, which can be imported into Geneious software for visualizing gene structures and boundaries. For cases where discrepancies were found between NCBI annotations and MITOS results, a Geneious-formatted validation file (2_geneious_alignment_validation_files.geneious) is provided, including manually corrected alignment evidence. The final compiled CSV file systematically organizes taxonomic information, gene rearrangement patterns (e.g., ND5-ND6 inversion clusters, tRNA t..., , , # Data on mitochondrial genome rearrangement patterns, annotation resources, and phylogenetic visualization in Actinopteri (ray-finned fishes)

Dataset DOI: [10.5061/dryad.m37pvmdd0](10.5061/dryad.m37pvmdd0)

## Description of the data and file structure

This dataset is suitable for studies on mitochondrial genome structural evolution, annotation pipeline validation, comparative genomics, and molecular phylogenetic analysis. All data comply with NCBI GenBank usage terms, involve no ethical concerns.

### Files and variables

#### File: 2\_geneious\_alignment\_validation\_files.geneious

**Description:** Manual verification process for discrepancies between NCBI and MITOS annotations.

#### File: 4\_Fish-phy.png

**Description:** Phylogenetic tree with annotated gene rearrangement events.

#### File: 0\_gb\_actinopteri\_14092\_from\_NCBI.gb

**Description:** Original mitochondrial genome records of ray-finned fishes downloaded from NCBI.

#### File: 1\_fasta\_bed\_actinopteri.zip

**Des...,

该数据集整合了辐鳍鱼类（Actinopterygii）线粒体基因组的结构变异、注释资源及系统发育分析结果。
原始数据来源于NCBI GenBank中公开的线粒体基因组记录（文件0_gb_actinopteri_14092_from_NCBI.gb），包含14092条原始序列。
通过MITOS在线工具生成标准化注释，得到包含FASTA序列与BED注释文件的压缩包（1_fasta_bed_actinopteri.zip），可导入Geneious软件以可视化基因结构及边界。
针对NCBI注释与MITOS结果存在差异的情况，提供了Geneious格式的验证文件（2_geneious_alignment_validation_files.geneious），内含人工校正的比对证据。
最终汇编的CSV文件系统整理了分类学信息、基因重排模式（如ND5-ND6倒位簇、tRNA t……，，，# 辐鳍鱼类线粒体基因组重排模式、注释资源及系统发育可视化数据）

数据集DOI：[10.5061/dryad.m37pvmdd0](10.5061/dryad.m37pvmdd0)

数据与文件结构描述

该数据集适用于线粒体基因组结构进化、注释流程验证、比较基因组学及分子系统发育分析等研究。所有数据符合NCBI GenBank使用条款，无伦理问题。

文件与变量

文件：2_geneious_alignment_validation_files.geneious
描述：NCBI注释与MITOS结果差异的人工验证过程。

文件：4_Fish-phy.png
描述：标注基因重排事件的系统发育树。

文件：0_gb_actinopteri_14092_from_NCBI.gb
描述：从NCBI下载的辐鳍鱼类原始线粒体基因组记录。

文件：1_fasta_bed_actinopteri.zip
描述：Des……