MicroRNA expression profiling in GC-2 cells following 50 Hz ELF-EMF. Mus musculus

Aberrant miRNA expression has been related to the development of human germ cell tumors, but little is known about effect of ELF-EMFs on miRNA expression. ELF-EMFs may epigenetically modify cells, which may account for the adverse effects of ELF-EMFs on the male reproductive system. To identify miRNAs that were differentially expressed between the sham and ELF-EMF exposure groups, we performed an Affymetrix microarray analysis to establish the miRNA expression profiles. Overall design: Mouse spermatocyte-derived GC-2 cells were intermittently exposed to a 50 Hz ELF-EMF for 72 h (5 min on/10 min off) at magnetic field intensities of 1 mT and 3 mT. miRNA expression was profiled using Affymetrix Mouse Genechip miRNA 3.0 arrays.

异常表达的微RNA（microRNA, miRNA）与人类生殖细胞肿瘤的发生发展密切相关，但目前关于极低频电磁场（Extremely Low Frequency Electromagnetic Fields, ELF-EMF）对miRNA表达的影响尚不清楚。极低频电磁场可通过表观遗传修饰细胞，这或许可以解释其对男性生殖系统产生的不良影响。为筛选出假照射组与极低频电磁场暴露组之间差异表达的miRNA，我们采用Affymetrix微阵列分析构建了miRNA表达谱。实验总体设计：以小鼠精母细胞来源的GC-2细胞为模型，采用50 Hz极低频电磁场进行72小时间歇性暴露（照射5分钟/暂停10分钟），磁场强度分别设置为1 mT与3 mT。采用Affymetrix小鼠基因芯片miRNA 3.0阵列对miRNA表达谱进行检测分析。