RNA-Seq of circCDYL knockdown (KD) samples in HepG2, J82, and UMUC3 cells and of GRWD1, IGF2BP1, and IGF2BP2 knockdown (KD) samples in J82 and UMUC3 cells

We profile gene expression upon circCDYL KD in HepG2 cells and in two bladder cancer cell lines J82 and UMUC3 as well as upon knockdown of the RNA binding proteins (RBP) GRWD1, IGF2BP1, and IGF2BP2 in J82 and UMUC3 Cells were treated with a control scramble (scr) siRNA or an siRNA that specifically knock down the expression of circCDYL or the RBP of interest. mRNA profiling upon treatment were generated using QuantSeq

本数据集针对HepG2细胞、两种膀胱癌细胞系J82及UMUC3的circCDYL敲低（KD）模型，以及J82、UMUC3细胞中RNA结合蛋白（RNA Binding Protein, RBP）GRWD1、IGF2BP1、IGF2BP2的敲低模型开展基因表达谱分析。所有受试细胞均接受了阴性对照乱序（scramble, scr）小干扰RNA（small interfering RNA, siRNA）处理，或特异性靶向敲低circCDYL或目标RBP表达的siRNA处理。各组处理后的mRNA表达谱通过QuantSeq测序技术完成构建。