Sequencing results of FTO overexpressing in papillary thyroid cells [meRIP-seq]

We tried to analyze the effect of FTO on papillary thyroid carcinoma. We constructed a FTO overexpression stable cell line of papillary thyroid carcinoma cells. We performed meRIP-seq sequencing analysis of the FTO overexpression stable transfer cell line to try to assess which genes were changed at the m6A level in papillary thyroid cancer cells by overexpressing FTO. Overall design: Comparative sequencing analysis of FTO overexpression stabilized cell lines of papillary thyroid carcinoma examined against normal papillary thyroid carcinoma cell lines.

本研究旨在探究脂肪量与肥胖相关蛋白（FTO）对乳头状甲状腺癌（papillary thyroid carcinoma）的作用效应。我们构建了乳头状甲状腺癌细胞的FTO过表达稳定细胞系，并针对该FTO过表达稳定转染细胞系开展了甲基化RNA免疫沉淀测序（meRIP-seq）分析，以明确过表达FTO后乳头状甲状腺癌细胞内哪些基因的N6-甲基腺嘌呤（m6A）修饰水平发生了改变。实验整体设计：以正常乳头状甲状腺癌细胞系为对照，对乳头状甲状腺癌FTO过表达稳定细胞系开展对比测序分析。