Supplementary Figure 30 and 34 Fluorescence microscopy and bright-field images of the 4-component assembly confirm incorporation of IgG-Alexa Fluor 568 or AtzBSH2 into assembly

Supp Figure 30: <b>Fluorescence microscopy and bright-field images of the 4-component assembly</b> (AtzAM1, AtzCM1, ProteinA-SH2, and antibody, along with extended linker versions of AtzA and AtzC) <b>confirm incorporation of IgG-Alexa Fluor 568 into assemblies.</b> (A) Fractal assembly in DIC and (B) fluorescent image of fractal indicating incorporation of antibody into assembly. (C) Globular assembly in DIC and (D) fluorescent images of globular assembly indicating incorporation of antibody into assembly. The depiction of a fractal and globular topology is easily distinguishable in these images. Supp Figure 34: <b>Fluorescence microscopy and bright-field images of the 3-component assembly confirm incorporation of AtzBSH2 into assembly while bright-field images confirm the fractal-like nature of the 2-component assembly. </b>(A and B) 3 µM AtzAM1, 1 µMAtzBSH2 dye labeled with Alexa FluorTM 647, 2 µM AtzCM1 image shows AtzBSH2 incorporation into 3-component assembly at various locations (C to H) 3 µM AtzAM1 and 2 µM AtzCM1 assembly images depict fractal-like assembly structure.<br>

补充图30：<b>四组分组装体的荧光显微镜成像与明场成像</b>（组分包含AtzAM1、AtzCM1、ProteinA-SH2以及抗体，同时附带AtzA与AtzC的延长接头版本）<b>证实Alexa Fluor 568标记的免疫球蛋白G（IgG-Alexa Fluor 568）成功整合进入该组装体。</b> (A) 微分干涉差（Differential Interference Contrast, DIC）成像下的分形组装体，(B) 对应分形组装体的荧光成像，表明抗体已整合进入组装体；(C) 微分干涉差成像下的球状组装体，(D) 对应球状组装体的荧光成像，证实抗体已整合进入组装体。从上述成像中可清晰区分分形与球状两种拓扑结构。
补充图34：<b>三组分组装体的荧光显微镜与明场成像证实AtzBSH2成功整合进入组装体，而明场成像则验证了二组分组装体具备分形样结构特征。</b> (A与B) 由3 μM AtzAM1、1 μM经Alexa Fluor™ 647标记的AtzBSH2以及2 μM AtzCM1构成的实验组成像结果显示，AtzBSH2在多个位点整合进入三组分组装体；(C至H) 由3 μM AtzAM1与2 μM AtzCM1构成的组装体成像呈现出分形样组装结构。