TMT-based proteomics analysis of GV, GVBD and MII oocytes

Two replicates of GV, GVBD, and MII oocytes were subjected to the 6-plex TMT labeling, HP-RP fractionation, and LC-MS/MS analysis. Two labeling experiments were performed for the total four replicates of each stage of oocytes.

针对生发泡（germinal vesicle, GV）期、生发泡破裂（germinal vesicle breakdown, GVBD）期以及第二次减数分裂中期（metaphase II, MII）卵母细胞，分别各取两份生物学重复样本，所有样本均接受了6联串联质量标签（tandem mass tag, TMT）标记、高效反相分级分离（high-performance reversed-phase fractionation, HP-RP）以及液相色谱-串联质谱（liquid chromatography-tandem mass spectrometry, LC-MS/MS）分析；针对每个卵母细胞发育阶段的全部四份重复样本，共开展了两次标记实验。