Vitamin D treatment timing is critical for transcriptome modulation of immune challenged primary human cells

In this study, the transcriptome of peripheral blood mononuclear cells (PBMCs) of a healthy adult was investigated in response to in vitro treatment with LPS, beta-glucan and 1,25(OH)2D3. RNA-seq of PBMCs of one individual treated immediately after isolation in 3 biologial repeats with 10 ng/ml lipopolysaccharide (L), 5 µg/ml beta-glucan (B), their solvent (0.1% DMSO (D)), the biologically active form of vitamin D, 10 nM 1,25(OH)2D3 (V) or its solvent (0.1% ethanol (E))

本研究针对一名健康成人的外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）的转录组展开分析，探究其经体外分别采用脂多糖（lipopolysaccharide, LPS）、β-葡聚糖（beta-glucan）以及1,25-二羟维生素D3[1,25(OH)2D3]处理后的应答反应。本研究对单一个体分离后即刻进行处理的PBMCs开展RNA测序（RNA-seq），共设置3次生物学重复，处理组别包括：10 ng/ml脂多糖（标注为L）、5 μg/ml β-葡聚糖（标注为B）、二者的溶剂对照（0.1%二甲基亚砜，DMSO，标注为D）、10 nM 1,25(OH)2D3（维生素D的生物活性形式，标注为V），以及该活性物质的溶剂对照（0.1%乙醇，标注为E）