Single cell gene expression profiling of CARD9 WT and R101C murine skin infected with Trichophyton rubrum

We used scRNA-seq to understand how CARD9 R101C remodels skin immunity and cell state in response to fungal infection in a mouse model of dermatophytosis. Overall design: We performed single cell RNA sequencing (scRNAseq) of skin from WT and CARD9 R101C mice infected with T. rubrum at day two (D2) and day nine (D9) post-infection (3 replicates of each WT and CARD9 R101C at each timepoint)

本研究采用单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）技术，以探究皮肤癣菌病小鼠模型中，CARD9 R101C突变如何重塑皮肤免疫与细胞状态以应对真菌感染。总体实验设计：我们对感染红色毛癣菌（T. rubrum）的野生型（Wild Type, WT）及CARD9 R101C突变型小鼠，分别于感染后第2天（D2）与第9天（D9）采集皮肤样本进行单细胞RNA测序；每个时间点下，WT组与CARD9 R101C组均设置3次生物学重复。