TCF7L1 controls tuft cell differentiation
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https://www.ncbi.nlm.nih.gov/sra/SRP424825
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资源简介：
To study the functions of Tcf7l1 during gut development, we have ablated Tcf7l1 specifically in the intestinal epithelium using Shh-Cre. To determine transcriptional changes upon loss of Tcf7l1, we have isolated EpCAM-positive epithelial cells from mouse embryos using fluorescence activated cell sorting (FACS) and performed RNA-sequencing analysis. Overall design: Samples: Shhcre-Egfp:Tcf7l1lox/lox and wild type. Cell type: epithelial cells from the small intestine of mouse embryos. Stage of embryogenesis: embryonic day 13.5 (E13.5). Replicates: three biological replicates.
为探究转录因子7类似物1（Tcf7l1）在肠道发育过程中的生物学功能，我们利用Shh-Cre工具在小鼠肠上皮细胞中特异性敲除了Tcf7l1基因。为明确Tcf7l1缺失后引发的转录组变化，我们通过荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）技术，从小鼠胚胎中分离出上皮细胞黏附分子（Epithelial Cell Adhesion Molecule, EpCAM）阳性上皮细胞，并开展了RNA测序（RNA-sequencing）分析。
实验整体设计：
样本：Shhcre-Egfp:Tcf7l1lox/lox基因工程小鼠与野生型小鼠
细胞类型：小鼠胚胎小肠上皮细胞
胚胎发育阶段：胚胎第13.5天（E13.5）
生物学重复：3次生物学重复
创建时间：
2023-06-16
