MOESM2 of Novel molecular components involved in callose-mediated Arabidopsis defense against Salmonella enterica and Escherichia coli O157:H7

Additional file 2. Relative gene expression analysis (mock- versus SL1344-treated leaves) using Log2 Fold Change and Z-ratio methods. Gene expression for each gene was calculated based on an average of three biological replicates. A total of 541 array probes corresponding to 585 genes were identified as differentially expessed based on Z-ratio analysis (the extreme 2% of up or down-regulated). Genes hilightes in bold had their expression evaluated by RT-qPCR.

附加文件2：采用对数2倍变化（Log2 Fold Change）与Z比率（Z-ratio）方法，针对空白对照（mock）处理与SL1344处理的叶片样本开展相对基因表达分析。每个基因的表达量均基于三次生物学重复的平均值计算得到。通过Z比率分析（筛选上调或下调表达的极端2%基因），共鉴定出对应585个基因的541个阵列探针为差异表达探针。以粗体标注的基因已通过实时定量聚合酶链式反应（RT-qPCR）验证了其表达水平。