Population growth rate data and scripts concerning the publication:"Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast"experiments

The data entails raw and processed data from population growth rates experiments using different yeast mutants. Moreover, it contains the required scripts to processthe data and reproduce Fig3 and Supplemental Figures 4 and 5 from the paper "Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast" published in Nature Communications.The experiments investigated the role of Cdc42 copy number in the polarization efficiency of different relevant mutants containing knockout mutations in Bem1 and Bem3 genes. The Cdc42 copy number was controlled using a galactose promoter.Initially the growth measurements were taken using a sfGFP tag fussed in Cdc42 (data shown in Fig3). Therefore, we did control experiments, shown in the Supplemental material, to check that the sfGFP tag was not influencing the results we observed previously (Supp Fig 4 and Supp Fig 5).<br><br><br>

该数据集包含不同酵母突变体种群增长率实验的原始与处理后数据。此外，它包含处理数据所需的脚本，以及重现发表于《自然通讯》（Nature Communications）的论文《冗余性与蛋白质拷贝数在出芽酵母细胞极化机制中的作用》中图3、补充图（Supplemental Figures）4和5的脚本。

这些实验研究了Cdc42拷贝数在含有Bem1和Bem3基因敲除突变的相关突变体极化效率中的作用。Cdc42拷贝数通过半乳糖启动子进行调控。

最初，使用融合在Cdc42上的sfGFP标签（sfGFP tag）进行生长测量（数据见图3）。因此，我们开展了补充材料（Supplemental material）中所示的对照实验，以验证sfGFP标签未对先前观察到的结果产生影响（补充图4和5）。