Transcriptomic analysis of H2S fumigated and Cys treated Arabidopsis seedlings

Numerous studies have revealed the gasotransmitter functions of hydrogen sulfide (H2S) in various biological processes. However, the involvement of H2S in sulfur metabolism and/or Cys synthesis makes its role as a signaling molecule ambiguous. The generation of endogenous H2S in plants is closely related to the metabolism of Cys, which play roles in a variety of signaling pathway occurring in various cellular processes. Here, we provided comprehensive transcriptomic analysis to support the gasotransmitter role of H2S besides as a substrate for Cys synthesis. Comparison of the differentially expressed genes (DEGs) between H2S and Cys treated seedlings indicated that H2S fumigation and Cys treatment caused different influences on gene profiles during seedlings development.

诸多研究已揭示硫化氢（hydrogen sulfide, H2S）作为气体信号分子在各类生物学过程中的功能。然而，由于硫化氢参与硫代谢及/或半胱氨酸（cysteine, Cys）合成，其作为信号分子的功能仍存在歧义。植物体内源性硫化氢的生成与半胱氨酸代谢紧密相关，而半胱氨酸参与各类细胞过程中的多种信号通路。本研究通过全面的转录组学分析，证实了硫化氢除作为半胱氨酸合成底物外，还可发挥气体信号分子的功能。通过对比硫化氢与半胱氨酸处理的幼苗的差异表达基因（differentially expressed genes, DEGs），本研究发现硫化氢熏蒸与半胱氨酸处理对幼苗发育过程中的基因表达谱产生了不同影响。